Clinical implications of differences between two recommended procedures for determination of aspartate aminotransferase.
نویسندگان
چکیده
We compared two officially recommended methods for determination of aspartate aminotransferase (EC 2.6.1.1): that of the International Federation of Clinical Chemistry (IFCC) and that of the Deutsche Gesellschaft für Klinische Chemie (DGKC). We used automated enzyme analyzers, initiating the reactions with 2-oxoglutarate. Normal values, 10-30 U/L (IFCC) and 7-18 U/L (DGKC), were apparently insensitive to intra-individual variations. Samples obtained from patients with heart disease showed a markedly different amount of activation with pyridoxal phosphate as compared with samples from other patient categories. Ratios for aspartate aminotransferase/alanine aminotransferase, as used in the differentiation of liver disease, can still be used with either method for determination of aspartate aminotransferase.
منابع مشابه
Evaluation of cardiac troponin I (cTnI), electrocardiography, creatine kinase (CK) and aspartate aminotransferase (AST) in diagnosis of selenium deficiency in Markhoz goat kids breed
BACKGROUND: Cardiac Troponin I (cTnI) is known as a cardiac biomarker in determining the myocardial damage of diseases which affect the heart muscle. OBJECTIVES: This study aims to evaluate the serum cTnI concentration in Markhoz Breed goats suffering selenium (Se) deficiency and its correlation with electrocardiographic parameters, and activity of creatine kinase (CK) and aspartate aminotransf...
متن کاملDetermination of serum aspartate aminotransferase with pyridoxal 5'-phosphate in the Technicon SMAC and Du Pont aca compared and correlated with the IFCC recommended method.
Inexpensive improvements in continuous-flow analytical apparatus can eliminate some perplexing inconsistencies and limitations in the use of the Technicon SMAC instrument for measuring the activity in serum of aspartate aminotransferase (EC 2.6.1.1) in the presence of pyridoxal 5'-phosphate. In addition, a calibration procedure based on values for this enzyme obtained by a modified IFCC method ...
متن کاملبررسی عوارض ناشی از مصرف استروئیدهای آنابولیک-آندروژنیک بر آنزیم‌های کبدی (ALT، AST، ALP) و فاکتورهای هماتولوژیک سرمی در مردان ورزشکار رشته‌ی پرورش اندام
Background and Objective: Effort for improving athletic function makes athletes use stimulants. The aim of the present study was to investigate the effects of consuming steroids on liver enzymes and blood factors in male bodybuilders. Materials and Methods: Ex post facto design comprising 48 male bodybuilders (age 25.02 ± 4.73 years old, weight 80.75 ± 6.33 kg) were assigned to three groups...
متن کاملThe Association of Physical Activity and Physical Fitness Levels with Liver Enzymes in COVID-19 Patients
Introduction: Exercise is one of the lifestyle interventions recommended to reduce liver damage and improve health. The present study aimed to investigate the relationship between physical activity and physical fitness with the liver enzymes of COVID-19 patients. Materials and Methods: Four hundred patients infected with COVID-19 (57.6±14.6, body mass 28.1±4.7 kg/m2) admitted to the Masih Danes...
متن کاملCommon reference intervals for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and γ-glutamyl transferase (GGT) in serum: results from an IFCC multicenter study.
BACKGROUND Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and γ-glutamyl transferase (GGT) measurements are important for the assessment of liver damage. The aim of this study was to define the reference intervals (RIs) for these enzymes in adults, paying attention to standardization of the methods used and careful selection of the reference population. METHODS AST, ALT and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 29 3 شماره
صفحات -
تاریخ انتشار 1983